Flow cytometric analysis of Mouse Ly-6G/Ly-6C expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b Antibody and either PE Rat IgG2b, κ Isotype Control (Left panel) or PE Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (S-R425)
PE Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (S-R425)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly6G |
| Synonyms | Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-R425 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
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