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PE Mouse Anti-human HLA-ABC Antibody (S-R526)

PE Mouse Anti-human HLA-ABC Antibody (S-R526)

Catalog Number: S0B5758 Application: FCM Reactivity: Human Conjugation: PE Brand: Starter
Price:
Regular price $95 USD
Regular price Sale price $95 USD
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Product Details

Product Specification


Host Mouse
Antigen HLA-ABC
Synonyms HLA class I histocompatibility antigen; A alpha chain; Human leukocyte antigen A (HLA-A); HLA class I histocompatibility antigen; B alpha chain; Human leukocyte antigen B (HLA-B); HLA class I histocompatibility antigen, C alpha chain; HLA-Cw; Human leukocyte antigen C
Location Cell membrane
Accession P04439、P01889、P10321
Clone Number S-R526
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Hu
Positive Sample human peripheral blood Leukocytes
Purification Protein A
Concentration 0.2 mg/ml
Conjugation PE
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Hu

Background

HLA-ABC (also termed HLA class I classical heavy chains A, B, and C) are 44 kDa type I transmembrane glycoproteins encoded within the major histocompatibility complex on chromosome 6p21.3; each non-covalently associates with the invariant light chain β2-microglobulin (12 kDa) to form a peptide-binding heterodimer that is constitutively expressed on nearly all nucleated cells. These molecules serve as the principal conduit for presenting endogenously derived 8–11-mer peptides to CD8⁺ cytotoxic T lymphocytes, thereby orchestrating immune surveillance against intracellular pathogens, tumors, and transplanted tissues. Polymorphic residues concentrated in the α1 and α2 domains create an extraordinary diversity (thousands of alleles catalogued in the IPD-IMGT/HLA database), which underpins population-level resistance to infection yet constitutes the primary barrier to solid-organ and hematopoietic stem-cell transplantation through allorecognition. Beyond antigen presentation, HLA-ABC interacts with NK-cell inhibitory receptors (KIR and LILRB1) to calibrate natural killer cell responses, and its surface levels are dynamically modulated by cytokines such as interferon-γ, viral immune evasion proteins (e.g., HIV Nef, CMV US2/US11), and oncogenic signaling pathways, making quantitative HLA-ABC expression a prognostic biomarker in cancer immunotherapy.

Picture

FC

Flow cytometric analysis of Human HLA-ABC expression on human peripheral blood Leukocytes. Human peripheral blood Leukocytes were stained with either PE Mouse IgG2a, κ Isotype Control (Black line histogram) or PE Mouse Anti-human HLA-ABC Antibody (Black line histogram) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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