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PE Mouse Anti-Human CD54 Antibody (S-3807)

PE Mouse Anti-Human CD54 Antibody (S-3807)

Catalog Number: S0B80085 Application: FCM Reactivity: Human Conjugation: PE Brand: Starter
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$135 USD
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Product Details

Product Specification


Host Mouse
Antigen CD54
Synonyms Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; ICAM1
Location Membrane
Accession P05362
Clone Number S-3807
Antibody Type Mouse mAb
Isotype IgG2a
Application FCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein A
Concentration 0.1 mg/ml
Conjugation PE
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1μl per million cells in 100μl volume Hu

Background

CD54 is an 85–110 kDa transmembrane glycoprotein of the immunoglobulin superfamily that is expressed on endothelial, epithelial and immune cells; upon binding its ligands LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) it serves as the critical adhesion checkpoint that promotes firm leukocyte arrest on the vessel wall, enables subsequent diapedesis into inflamed tissue, and amplifies antigen-specific T-cell activation, thereby positioning it at the mechanistic center of inflammatory, autoimmune and anti-tumor immune responses.

Picture

FC

Flow cytometric analysis of human CD54 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD19 Antibody and either PE Mouse IgG2a Isotype Control (left panel) or SDT PE Mouse Anti-Human CD54 Antibody (right panel) at 1 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.