Flow cytometric analysis of CD3d expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 Antibody and either PE Mouse IgG2a Isotype Control (left panel) or SDT PE Mouse Anti-Human CD3d Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PE Mouse Anti-Human CD3d Antibody (MEM-57)
PE Mouse Anti-Human CD3d Antibody (MEM-57)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD3d |
| Location | Cell membrane |
| Accession | P04234 |
| Clone Number | MEM-57 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Isotype Control | S0B8530 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD3d (CD3-delta) is a 19 kDa transmembrane glycoprotein that together with CD3ε, CD3γ and CD3ζ forms the CD3 signaling complex, which is tightly associated with the T-cell receptor (TCR) on thymocytes and mature T lymphocytes; upon antigen/MHC engagement the CD3 chains become phosphorylated on their cytoplasmic ITAMs by Lck and Fyn, initiating the cascade that leads to T-cell activation, proliferation and differentiation, while during thymic development CD3d is indispensable for correct assembly and surface expression of the pre-TCR/TCR complex and thus for positive selection of CD4⁺ and CD8⁺ T cells.
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