Flow cytometric analysis of Mouse Ly-6G/Ly-6C (Gr-1) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either PE-Cy7 Rat IgG2b, κ Isotype Control (Left panel) or SDT PE-Cy7 Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (Right panel) at 1.25μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE-Cy7 Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (S-R425)
PE-Cy7 Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (S-R425)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly6G |
| Synonyms | Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-R425 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
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