Flow cytometric analysis of Mouse CD23 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either PE-Cy7 Rat IgG2a, κ Isotype Control (left panel) or SDT PE-Cy7 Rat Anti-Mouse CD23 Antibody (right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PE-Cy7 Rat Anti-Mouse CD23 Antibody (S-R672)
PE-Cy7 Rat Anti-Mouse CD23 Antibody (S-R672)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD23 |
| Location | Secreted, Cell membrane |
| Accession | P20693 |
| Clone Number | S-R672 |
| Antibody Type | Rat mAb |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD23, also known as the low-affinity IgE receptor (FcεRII), is a 45 kDa type II integral membrane glycoprotein that plays a crucial role in regulating IgE production and B-cell differentiation. It is expressed on various immune cells, including mature B cells, monocytes, eosinophils, follicular dendritic cells, and platelets. CD23 exists in two isoforms, CD23a and CD23b, which differ in their amino acid sequences and functions. The molecule can also be cleaved into a soluble form (sCD23) that retains IgE-binding capabilities.
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