Flow cytometric analysis of CD15 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE-Cy7 Mouse IgM,k Isotype Control (left panel) or SDT PE-Cy7 Mouse Anti-Human CD15 Antibody (right panel) at 5 μl/test with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PE-Cy7 Mouse Anti-Mouse/Human CD15 Antibody (MC-480)
PE-Cy7 Mouse Anti-Mouse/Human CD15 Antibody (MC-480)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD15 |
| Synonyms | Stage-Specific Embryonic Antigen 1; X-hapten; Lewis X; 3-FAL; SSEA1; SSEA-1 |
| Location | Cell membrane |
| Clone Number | MC-480 |
| Antibody Type | Mouse mAb |
| Isotype | IgM,k |
| Application | FCM |
| Reactivity | Hu, Ms |
| Positive Sample | Human peripheral blood cells |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Hu, Ms |
Background
CD15, also known as Lewis X or SSEA-1, is a carbohydrate epitope (Galβ1→4[Fucα1→3]GlcNAc) carried primarily on glycoproteins and glycolipids of myeloid cells, granulocytes, and certain lymphocyte subsets; it serves as a key adhesion molecule that mediates selectin-dependent leukocyte rolling during inflammation, acts as a marker of mature neutrophils and Hodgkin/Reed-Sternberg cells, and its differential expression is exploited in immunohistochemistry to distinguish myeloid leukemias from lymphoid malignancies and to identify stem-cell populations in normal and neoplastic tissues.
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