Flow cytometric analysis of Mouse CD45.1 expression on SJL mouse splenocytes. SJL mouse splenocytes were stained either PE-Cy7 Mouse IgG2a, κ Isotype Control (left panel) or SDT PE-Cy7 Mouse Anti-Mouse CD45.1 Antibody (right panel) at 1.25 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE-Cy7 Mouse Anti-Mouse CD45.1 Antibody (A20)
PE-Cy7 Mouse Anti-Mouse CD45.1 Antibody (A20)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD45.1 |
| Location | Cell membrane |
| Accession | P06800 |
| Clone Number | A20 |
| Antibody Type | Mouse mAb |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | SJL mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 0.25μg per million cells in 100μl volume | Ms |
Background
CD45.1 is a protein isoform of CD45, also known as PTPRC, which is a receptor-type tyrosine phosphatase expressed on all hematopoietic cells except erythrocytes and platelets. This type I transmembrane protein plays a crucial role in the activation processes of B and T cell receptors, as well as in thymic selection. CD45.1, along with CD45.2, is commonly used in adoptive cell transfer experiments to distinguish donor cells from host cells due to their allelic differences. Mice carrying the CD45.1 allele, such as C57BL/6J, are widely used in research for this purpose. CD45.1 is also used in studies to understand the rejuvenating effects of young blood on aged tissues. In heterochronic parabiosis models, where the circulatory systems of old and young mice are connected, CD45.1 and CD45.2 genotypes are used to track the cells of old and young mice, respectively.
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