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PE-Cy7 Mouse Anti-Human CD8α Antibody (OKT-8)

PE-Cy7 Mouse Anti-Human CD8α Antibody (OKT-8)

Catalog Number: S0B80640 Application: FCM Reactivity: Hu Conjugation: PE-Cy7 Brand: Starter
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$85 USD
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Product Details

Product Specification


Host Mouse
Antigen CD8α
Synonyms T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; MAL; CD8A
Location Secreted, Cell membrane
Accession P01732
Clone Number OKT-8
Antibody Type Mouse mAb
Isotype IgG2a
Application FCM
Reactivity Hu
Positive Sample Human peripheral blood leukocytes
Purification Protein A
Concentration 0.1 mg/ml
Conjugation PE-Cy7
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 2μl per million cells in 100μl volume Hu

Background

CD8α is a crucial immune glycoprotein that plays a vital role in the immune response by serving as a co-receptor for the T-cell receptor (TCR) on cytotoxic T cells. It forms either a homodimer (α/α) or a heterodimer with CD8β (α/β) to interact with MHC class I molecules on antigen-presenting cells (APCs), facilitating the recognition of intracellular pathogens such as viruses. CD8α also recruits the LCK kinase, which initiates downstream signaling pathways essential for T-cell activation and proliferation. This molecule is critical for the function of cytotoxic T lymphocytes (CTLs) and is involved in various immune-related diseases, including immunodeficiencies and autoimmune conditions.

Picture

FC

Flow cytometric analysis of CD8α expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD4 Antibody and either PE-Cy7 Mouse IgG2a, κ Isotype Control (left panel) or SDT PE-Cy7 Mouse Anti-Human CD8α Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.