WB result of PDK1/PDPK1 Recombinant Rabbit mAb
Primary antibody: PDK1/PDPK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: 22Rv1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 55-65 kDa
PDK1/PDPK1 Recombinant Rabbit mAb (S-3050-116)
PDK1/PDPK1 Recombinant Rabbit mAb (S-3050-116)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PDK1/PDPK1 |
| Synonyms | 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | O15530 |
| Clone Number | S-3050-116 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, Jurkat, HeLa, K562, MCF7, 22Rv1, NIH/3T3, mouse testis, PC-12, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
PDK1 (officially PDPK1), the 3-phosphoinositide-dependent protein kinase-1, is a 556-aa serine/threonine kinase whose N-terminal catalytic domain followed by a C-terminal PH domain that binds PtdIns(3,4,5)P3 and PtdIns(3,4)P2 generated by PI3K; once recruited to the plasma membrane it uses a docking-site motif (termed PIF-pocket) to phosphorylate the T-loop of AGC kinases such as AKT, S6K, SGK and PKC isoforms, thereby serving as the master upstream activator of the PI3K–AKT–mTOR signaling axis that controls cell growth, proliferation, survival and metabolism, while its dysregulation through PTEN loss, PI3K mutation or PDK1 overexpression is a frequent driver of cancer and metabolic disorders.
Picture
Picture
Western Blot
WB result of PDK1/PDPK1 Recombinant Rabbit mAb
Primary antibody: PDK1/PDPK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 55-65 kDa
WB result of PDK1/PDPK1 Recombinant Rabbit mAb
Primary antibody: PDK1/PDPK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 55-65 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- PDK1/PDPK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
