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PDHE1 Alpha Mouse mAb

PDHE1 Alpha Mouse mAb

Catalog Number: S0B60301 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen PDHE1 Alpha
Synonyms Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A; PDHA1
Location Mitochondrion
Accession P08559
Antibody Type Mouse mAb
Isotype IgG2a
Application WB, IHC-P
Reactivity Hu, Ms, Rt
Positive Sample HepG2, K-562, Jurkat, NIH/3T3, mouse brain, rat brain
Purification Protein A
Concentration 1 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:5000-1:50000 Hu, Ms, Rt
IHC-P 1:400-1:1600 Hu, Ms, Rt

Background

PDHE1 Alpha (i.e., pyruvate dehydrogenase E1 subunit alpha, encoded by the PDHA1 gene) is the catalytic core subunit of the first enzyme component (E1) of the pyruvate dehydrogenase complex (PDC), located in the mitochondrial matrix. Through its thiamine pyrophosphate (TPP) cofactor-dependent active site, this protein catalyzes the oxidative decarboxylation of pyruvate to generate a hydroxyethyl-TPP intermediate, which is a critical irreversible step that channels the glycolytic product pyruvate into the tricarboxylic acid (TCA) cycle, thus occupying a central position in aerobic glucose oxidation across the entire cell. The activity of PDHE1 Alpha is finely regulated by a complex phosphorylation/dephosphorylation cycle: specific pyruvate dehydrogenase kinases (PDKs) inactivate it by phosphorylating three serine residues (especially Ser-293), while pyruvate dehydrogenase phosphatases (PDPs) restore its activity through dephosphorylation, allowing flexible regulation of metabolic flux according to substrate and energy supply-demand status. The PDHA1 gene encoding this protein is located on the X chromosome, and its pathogenic mutations are among the most common genetic causes of Leigh syndrome, lactic acidosis, and various early-onset neurodegenerative diseases, with patients often presenting with developmental delay, dystonia, and neurological dysfunction—highlighting the special importance of this protein in maintaining cerebral energy metabolism.

Picture

Western Blot

WB result of PDHE1 Alpha Mouse mAb
Primary antibody: PDHE1 Alpha Mouse mAb at 1/20000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 39 kDa

WB result of PDHE1 Alpha Mouse mAb
Primary antibody: PDHE1 Alpha Mouse mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 39 kDa


WB result of PDHE1 Alpha Mouse mAb
Primary antibody: PDHE1 Alpha Mouse mAb at 1/20000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 39 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human heart muscle. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse heart muscle. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat heart muscle. Anti-PDHE1 Alpha antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.