The PDE4A4B activity was assayed by FP technology. The reaction was performed by incubating the PDE4A4B protein, substrate at 25℃ for 60 min, and adding the binding agent at 25℃ for 60 min, then reading FP signal with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | DPDE2 |
Accession | P27815-1 |
Amino Acid Sequence | E2-T886(end), with N-terminal GST tag&C-terminal Flag tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 125.9 kDa |
Purity | >80% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | GST Tag, Flag Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 10% Glycerol, 1 mM DTT, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
