WB result of PDE4 Recombinant Rabbit mAb
Primary antibody: PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Negative control: K562 whole cell lysate; DU 145 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
PDE4 Recombinant Rabbit mAb (S-2598-147)
PDE4 Recombinant Rabbit mAb (S-2598-147)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PDE4 |
| Synonyms | 3',5'-cyclic-AMP phosphodiesterase 4A; DPDE2; PDE46; cAMP-specific phosphodiesterase 4A; PDE4A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P27815 |
| Clone Number | S-2598-147 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SH-SY5Y, mouse brain, mouse cerebellum, rat brain, rat cerebellum |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:100 | Hu, Ms, Rt |
Background
PDE4 (phosphodiesterase-4) is a cAMP-specific phosphodiesterase that hydrolyzes the second messenger cyclic adenosine monophosphate (cAMP) to 5'-AMP, thereby terminating cAMP signaling cascades involved in inflammation, memory, cardiac contractility and immune cell regulation; the enzyme family comprises four subtypes (PDE4A-D) that differ in N-terminal targeting domains and regulatory Upstream Conserved Regions (UCR1/2) controlling dimerization and PKA-mediated phosphorylation, and selective inhibition of PDE4—for example with roflumilast or apremilast—elevates intracellular cAMP, suppressing pro-inflammatory cytokines (TNF-α, IL-23) and increasing anti-inflammatory IL-10, making PDE4 inhibitors clinically useful in psoriasis, psoriatic arthritis, Behçet’s disease and severe COPD.
Picture
Picture
Western Blot
WB result of PDE4 Recombinant Rabbit mAb
Primary antibody: PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 80-110 kDa
WB result of PDE4 Recombinant Rabbit mAb
Primary antibody: PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 80-100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PDE4 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-PDE4 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PDE4 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
