Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 1.5μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with an EC50 of 0.141-0.174µg/mL.
PD-1 His Tag Protein, Human
PD-1 His Tag Protein, Human
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$180 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Programmed cell death-1, CD279, Programmed death receptor 1 |
| Accession | Q15116-1 |
| Amino Acid Sequence | Leu25-Gln167, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 28-36 kDa (Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
PD-1 (programmed cell death-1) is a transmembrane protein important for regulating immune responses. It is a member of the CD28/CTLA-4 family of immunoreceptors and is expressed on T cells and pro-B cells suggesting it has a broad spectrum of immune regulation. PD-1 along with its two ligands, PD-L1 and PD-L2, prevent the activation of T cells and protect tissues from autoimmune attack. PD-1 and its ligands are also involved in reduction of infectious immunity, as well as tumor immunity. PD-1 has been shown to facilitate chronic infection and tumor progression. Drugs targeting PD-1 have shown promise in treating cancer and HIV and may be important in a variety of immune related disorders.
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Bioactivity
Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 0.5μg/mL (100μL/well) can bind Nivolumab (starter-bio, Cat. No. S0B0566) with EC50 of 2.24-2.98 ng/mL.
Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 0.5μg/mL (100μL/well) can bind Pembrolizumab (starter-bio, Cat. No. S0B0233) with EC50 of 1.28-1.49 ng/mL.
SDS-PAGE
PD-1 His Tag, Human ,1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.
SPR
PD-1 His Tag, Human (Cat. No. UA010004) captured on CM5 chip via anti-His antibody, can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with an affinity constant of 0.6644 μM as determined in a SPR assay (Biacore T200).
Loaded PD-L1 Fc Chimera, Human (Cat. No. UA010003) on Protein A Biosensor, can bind PD-1 His Tag, Human (Cat. No. UA010004) with an affinity constant of 1.6 μM as determined in BLI assay.
