Starter Magnetic Bead-Based PCR/DNA Purification and Recovery Kit

The Stater Magnetic Bead-based PCR Product & DNA Purification and Recovery Kit is a kit that uses novel magnetic beads coated with a nucleic acid purification medium, enabling stable, efficient, and convenient extraction, purification, and recovery of DNA from PCR products or various DNA reaction systems.

This kit is suitable for removing impurities such as primers, enzymes, mineral oil, glycerol, and salts after PCR reactions; it is also applicable to DNA purification following restriction digestion, ligation, phosphorylation, blunt-end repair, or random primer reactions. The purified DNA obtained can be directly used in subsequent experiments such as restriction digestion, ligation, transformation, sequencing, PCR, and hybridization.

Instructions for Use

1.This kit provides the corresponding reagents and magnetic beads based on a standard of50µL PCR/DNAsample purification and recovery.

Table4Recommended Usage of Magnetic Beads and Reagents for Different Sample Volumes

2.Activate the magnetic beads: according to the number of samples (n) and the recommended amount of magnetic beads in Table 4, take out the corresponding volume of magnetic bead solution (5n), and add an equal volume of elutionliquid(Eluent)Mix thoroughly, use a magnetic separation device to remove the wash liquid, repeat the above operation once, then add an equal volume of elution buffer.Elutionbuffer(Eluent)Mix the magnetic beads well and set aside for later use.

3.Add separately into the sample tube300µLof binding buffer(Binding Buffer)and 5 µL of activated magnetic beads, mix thoroughly by pipetting, let stand at room temperature for 5 minutes to allow the magnetic beads to fully bind with the nucleic acids (if necessary, the binding time can be appropriately extended to increase the yield).

4.Place the sample tube in the magnetic field of the magnetic stand. After the magnetic beads have completely aggregated, carefully aspirate the remaining liquid, being careful not to aspirate the magnetic beads, as this may affect the subsequent recovery yield.

5.Add300 µlWash Buffer: Use a pipette to mix and disperse the magnetic beads. Place the sample tube in the magnetic field of the magnetic stand. After the magnetic beads have completely aggregated, carefully remove any remaining liquid, avoiding aspirating the beads to prevent affecting the subsequent recovery yield.

6.Repeat step 5, trying to remove as much residual liquid as possible. Allow it to sit at room temperature for 3–5 minutes to ensure that any remaining ethanol has completely evaporated, so as not to affect subsequent experiments.

7.Add30–50 µlof elution buffer, use a pipette to mix well, suspending the magnetic beads in the solution, and incubate at room temperature for 2 minutes. Place the sample tube in the magnetic field; after the magnetic beads have completely aggregated, carefully transfer the supernatant to a new centrifuge tube and store it at-20°C. The resulting solution is the DNA sample.

Note: To increase the sample concentration, you may also reduce the volume of elution buffer appropriately, but it should not be less than3Double the volume to avoid incomplete elution. Raising the temperature of the elution buffer to 65°C in a metal bath or water bath can slightly improve elution efficiency compared to room-temperature elution.

1. When using this kit, you will need to provide your own anhydrous ethanol and a magnetic separation device.