PCNA Recombinant Rabbit mAb (S-5313)

Catalog Number: S0B60151 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	PCNA
Synonyms	DNA sliding clamp PCNA; Cyclin; Proliferating cell nuclear antigen (PCNA)
Location	Nucleus
Accession	P12004
Clone Number	S-5313
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, MCF7, 293T, HepG2, A431, NIH/3T3, C2C12, PC-12, C6, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:5000	Hu, Ms, Rt
ICC	1:500	Hu, Ms, Rt

Background

PCNA, or Proliferating Cell Nuclear Antigen, is a highly evolutionarily conserved homotrimeric ring-shaped protein composed of 261 amino acids with a molecular weight of approximately 29 kDa. Its three-dimensional structure presents as a closed circular "sliding clamp" with a central channel that can encircle and slide along double-stranded DNA. As a core component of the eukaryotic DNA replication machinery, PCNA serves as the processivity factor for DNA polymerases δ and ε during DNA replication, significantly enhancing their synthetic efficiency by anchoring the polymerases to the template strand. Simultaneously, it functions as a critical platform protein that bridges DNA replication, cell cycle regulation, and DNA damage repair networks. Through multiple protein interaction interfaces on its surface, PCNA can recruit and coordinate over dozens of different binding partners, including DNA ligase I, DNA methyltransferases, histone acetyltransferases, and the cyclin-dependent kinase (CDK) inhibitor p21. Notably, PCNA expression and synthesis peak during the S phase of the cell cycle, making it widely used as an immunohistochemical marker for cell proliferation status (e.g., as a complementary indicator to Ki-67). In response to DNA damage, specific lysine residues on PCNA undergo post-translational modifications such as ubiquitination and SUMOylation to switch its functional mode, shifting from supporting conventional replication to participating in DNA repair pathways such as translesion synthesis or template switching. Given that PCNA is highly expressed in actively proliferating tumor cells and is essential for cancer cell survival, inhibitors targeting PCNA—such as cyclic peptides or small-molecule compounds—are being actively developed as potential broad-spectrum anticancer agents.

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Western Blot

WB result of PCNA Recombinant Rabbit mAb
Primary antibody: PCNA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDa

WB result of PCNA Recombinant Rabbit mAb
Primary antibody: PCNA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDa

WB result of PCNA Recombinant Rabbit mAb
Primary antibody: PCNA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDa

WB result of PCNA Recombinant Rabbit mAb
Primary antibody: PCNA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-PCNA antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in 293T cells. Anti- PCNA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ICC shows positive staining in C2C12 cells. Anti- PCNA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ICC shows positive staining in C6 cells. Anti- PCNA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).