Flow cytometric analysis of PCNA expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with Alexa Fluor® 488 Rabbit IgG Isotype Control (Black line histogram) and SDT PCNA Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PCNA Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-297-116)
PCNA Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-297-116)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PCNA |
| Synonyms | Proliferating cell nuclear antigen; Cyclin |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P12004 |
| Clone Number | SDT-297-116 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa |
| Predicted Reactivity | Av, Bv, Hm |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | Hu |
| ICFCM | 1:800 | Hu |
Background
Proliferating cell nuclear antigen (PCNA) is a type of DNA clamp that acts as a DNA polymerase in eukaryotic cells δ. The processing factor of is crucial for replication. PCNA is a homologous trimer that achieves its processability by surrounding DNA, where it serves as a scaffold for recruiting proteins involved in DNA replication, DNA repair, chromatin remodeling, and epigenetics. It is expressed in most tumors as a proliferative antigen.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- PCNA (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
