WB result of PBX1 Recombinant Rabbit mAb
Primary antibody: PBX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 38, 47 kDa
PBX1 Recombinant Rabbit mAb (S-4698-8)
PBX1 Recombinant Rabbit mAb (S-4698-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PBX1 |
| Synonyms | Pre-B-cell leukemia transcription factor 1; Homeobox protein PBX1; Homeobox protein PRL; PRL |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P40424 |
| Clone Number | S-4698-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HeLa, NIH/3T3, mouse brain, mouse lung, rat brain, rat lung |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
Background
PBX1 (PBX homeobox 1) is a nuclear transcription factor belonging to the TALE/PBX homeobox family. It functions by binding to specific DNA sequences (such as 5'-ATCAATCAA-3') and regulating gene expression, often in complex with MEIS and HOX proteins. PBX1 plays crucial roles in embryonic development, including skeletal patterning, osteogenesis, and sexual differentiation. It is strongly expressed in tissues like the kidney and brain, and its dysregulation, notably through the t(1;19) chromosomal translocation, is closely associated with pre-B-cell acute lymphoblastic leukemia.
Picture
Picture
Western Blot
WB result of PBX1 Recombinant Rabbit mAb
Primary antibody: PBX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 38, 47 kDa
WB result of PBX1 Recombinant Rabbit mAb
Primary antibody: PBX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat lung lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 38, 47 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-PBX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-PBX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
