WB result of PBEF/Visfatin/NAMPT Rabbit pAb
Primary antibody: PBEF/Visfatin/NAMPT Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 54 kDa
PBEF/Visfatin/NAMPT Rabbit Polyclonal Antibody
PBEF/Visfatin/NAMPT Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PBEF/Visfatin/NAMPT |
| Synonyms | Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1 (Pre-B cell-enhancing factor); Visfatin; PBEF1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P43490 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, HT-1080, K562, MCF7, PC-3 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu |
Background
PBEF/Visfatin/NAMPT is a multifunctional 52-kDa protein that acts both intracellularly as the rate-limiting enzyme nicotinamide phosphoribosyltransferase (EC 2.4.2.12) in the NAD salvage pathway—catalyzing conversion of nicotinamide to nicotinamide mononucleotide (NMN)—and extracellularly as an adipokine/cytokine circulating in blood; it enhances B-lymphocyte precursor maturation, inhibits neutrophil apoptosis, binds and activates the insulin receptor to exert insulin-mimetic glucose-lowering effects, promotes vascular smooth-muscle cell maturation, modulates endothelial function, and its serum levels correlate with obesity and metabolic syndrome, while intracellularly it maintains the NAD⁺ pool essential for processes such as SIRT1 activity, DNA repair and β-cell glucose-stimulated insulin secretion.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
