WB result of Parvalbumin Recombinant Rabbit mAb
Primary antibody: Parvalbumin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: mouse skeletal muscle lysate 5 µg
Lane 2: mouse cerebellum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 14 kDa
Parvalbumin Recombinant Rabbit mAb (S-1620-10)
Parvalbumin Recombinant Rabbit mAb (S-1620-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Parvalbumin |
| Synonyms | Parvalbumin alpha, PVALB |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm |
| Accession | P20472 |
| Clone Number | S-1620-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Human cerebellum, mouse skeletal muscle, mouse cerebellum, rat skeletal muscle, rat cerebellum |
| Predicted Reactivity | Mk, Bv, Rb, Hm, Du, Ct |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:20000 | Ms, Rt |
| IHC-P | 1:500-1:2000 | Hu, Ms, Rt |
| IF | 1:100-1:500 | Hu, Ms, Rt |
Background
Parvalbumin is a small calcium-binding protein that belongs to the EF-hand protein family. It is expressed in vertebrates and is typically found in high concentrations in certain skeletal muscle fibers and neurons. Parvalbumin plays a crucial role as a mobile cytosolic Ca2+ buffer, influencing the duration of intracellular Ca2+ signals and is key in muscle relaxation following contraction and neuronal recovery following excitation. In the context of neuroscience, parvalbumin is highly expressed in the nervous system during development and is associated with the maturation of functional circuits. It is predominantly found in a specific subgroup of cortical interneurons and is involved in the inhibition of neuronal activity. Parvalbumin-expressing neurons are known for their fast-spiking behavior and are involved in various neurological processes, including the regulation of neuronal circuits and the modulation of synaptic transmission.
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Western Blot
WB result of Parvalbumin Recombinant Rabbit mAb
Primary antibody: Parvalbumin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: rat skeletal muscle lysate 5 µg
Lane 2: rat cerebellum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 14 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebellum. Anti- Parvalbumin antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebellum. Anti- Parvalbumin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebellum. Anti- Parvalbumin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
