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PARP2, GST Tag Protein

PARP2, GST Tag Protein

Catalog Number: UA080019 Brand: UA BIOSCIENCE
Price:
Regular price $1,120 USD
Regular price Sale price $1,120 USD
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Product Details

Product Specification


Species Human
Synonyms Poly [ADP-ribose] polymerase 2,PARP-2,hPARP-2,ADP-ribosyltransferase diphtheria toxin-like 2,ARTD2,DNA ADP-ribosyltransferase PARP2,NAD(+) ADP-ribosyltransferase 2,ADPRT-2,Poly[ADP-ribose] synthase 2,pADPRT-2,Protein poly-ADP-ribosyltransferase
Accession Q9UGN5
Amino Acid Sequence A2-W583(end)
Expression System Baculovirus-InsectCells
Molecular Weight

92.4 kDa

Purity >80% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Tag GST Tag
Physical Appearance Liquid
Storage Buffer 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Stability & Storage

Stable for 12 monthsupon stored at -80℃ from the date of receipt. And avoid repeated freeze-thawscycles.

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Bioactivity

The PARP2 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP2 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.

SDS-PAGE