WB result of PAPSS1 Recombinant Rabbit mAb
Primary antibody: PAPSS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A673 whole cell lysate 20 µg
Lane 6: Ramos whole cell lysate 20 µg
Low expression control: THP-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
PAPSS1 Recombinant Rabbit mAb (S-3516-9)
PAPSS1 Recombinant Rabbit mAb (S-3516-9)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PAPSS1 |
| Synonyms | Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1Alternative nameSulfurylase kinase 1 (SK 1; SK1); ATPSK1; PAPSS |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | O43252 |
| Clone Number | S-3516-9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, SK-OV-3, HepG2, A673, Ramos |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu |
Background
PAPSS1 (3'-Phosphoadenosine-5'-Phosphosulfate Synthase 1) is a key rate-limiting enzyme in the sulfation metabolic pathway, primarily responsible for catalyzing the synthesis of the active sulfate donor PAPS (3'-phosphoadenosine-5'-phosphosulfate). This protein consists of an N-terminal domain and a C-terminal domain: the N-terminal domain possesses ATP sulfurylase activity, catalyzing the reaction of sulfate with ATP to generate adenosine-5'-phosphosulfate (APS); the C-terminal domain exhibits APS kinase activity, further phosphorylating APS to produce PAPS. PAPSS1 is expressed at significantly higher levels in the brain compared to other tissues, suggesting its particularly important role in sulfation modifications within the nervous system, participating in the regulation of neurotransmitter metabolism, hormone activity, and the sulfation status of extracellular matrix components. The PAPS product of this enzyme is the most important active sulfate group donor in living organisms, providing substrates for various sulfotransferases and driving the sulfation of hundreds of compounds, including proteoglycans, glycolipids, hormones, and exogenous substances (drugs or toxins). These modifications are crucial for maintaining cell-cell communication, signal transduction, and detoxification processes. Clinical studies have found that mutations in the PAPSS1 gene are associated with a rare form of bone dysplasia, while PAPSS1 is aberrantly expressed in various tumors such as hepatocellular carcinoma and colorectal cancer, potentially promoting malignant progression by affecting the sulfation microenvironment of tumor cells.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-PAPSS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PAPSS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PAPSS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PAPSS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PAPSS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PAPSS1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Ramos cells. Anti-PASPSS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
