PAPP-A Recombinant Rabbit mAb (SDT-226-46)

Catalog Number: S0B0062 Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100 μl

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Product Details

Product Specification

Host	Rabbit
Antigen	PAPP-A
Synonyms	Pappalysin-1, IGFBP-4ase
Immunogen	Recombinant Protein
Accession	Q13219
Clone Number	SDT-226-46
Antibody Type	Rabbit mAb
Reactivity	Hu
Purification	Protein A
Concentration	0.25 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:1000	null

Background

PAPP-A is a proteolytic enzyme that was discovered to increase local insulin-like growth factor (IGF) availability for receptor activation through cleavage of inhibitory IGF binding proteins (IGFBPs). Reduced IGF signaling has been associated with increased lifespan and healthspan.
Therefore, inhibition of PAPP-A represents a novel approach to indirectly decrease the availability of bioactive IGF [PMID: 28035757IF: 8.0 Q1 B1].

Picture

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human placenta. Anti-PAPP-A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-PAPP-A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-PAPP-A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-PAPP-A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-PAPP-A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.