WB result of Pan Trk Rabbit mAb
Primary antibody: Pan Trk Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse kidney lysate; mouse spleen lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 120 kDa
Pan Trk Recombinant Rabbit mAb (SDT-400-39)
Pan Trk Recombinant Rabbit mAb (SDT-400-39)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Pan Trk |
| Synonyms | Trk A + Trk B + Trk C |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | P04629、Q16620、Q16288 |
| Clone Number | SDT-400-39 |
| Antibody Type | Rabbit mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Av |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:250 |
Background
Neurotrophic tyrosine kinase receptor (NTRK) is a family of 3 proto-oncogenes including NTRK1, NTRK2, and NTRK3 which encode Trk A, Trk B, and Trk C proteins, respectively [PMID: 28719467]. Pan-TRK antibodies have been used to detect gene fusions in diverse types of tumors [PMID: 37017998].
Picture
Picture
Western Blot
WB result of Pan Trk Rabbit mAb
Primary antibody: Pan Trk Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Lane 3: rat brain lysate 20 µg
Negative control: rat kidney lysate; rat spleen lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 120 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse astrocytoma. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
