pan PDE4 Recombinant Rabbit mAb (S-5034)

Catalog Number: S0B6942 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	pan PDE4
Synonyms	3',5'-cyclic-AMP phosphodiesterase 4D; DPDE3; PDE43; cAMP-specific phosphodiesterase 4D; PDE4D
Location	Cell membrane, Cytoplasm, Cytoskeleton
Accession	Q08499
Clone Number	S-5034
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, mouse brain, mouse cerebellum, rat brain, rat cerebellum
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu, Ms, Rt

Background

PDE4 is a member of the phosphodiesterase 4 family, belonging to the 3',5'-cyclic nucleotide-specific phosphodiesterases. Its primary function is to specifically hydrolyze the second messenger cyclic AMP (cAMP), converting it into inactive 5'-AMP, thereby terminating cAMP-mediated cell signaling pathways. The PDE4 family comprises four subtypes (PDE4A, PDE4B, PDE4C, PDE4D), each generating multiple variants through alternative splicing, with N-terminal targeting domains determining their subcellular localization and interactions with signaling complexes. As an important negative regulator, PDE4 controls local cAMP concentrations, thereby regulating downstream protein kinase A (PKA) and Epac signaling pathways, which in turn affect processes such as cell contraction, migration, proliferation, inflammatory responses, and learning and memory. Due to its high expression in immune and inflammatory cells, PDE4 has become an important therapeutic target for chronic inflammatory diseases, with selective inhibitors (e.g., apremilast, roflumilast) developed for the treatment of psoriasis, ankylosing spondylitis, and chronic obstructive pulmonary disease (COPD). Additionally, PDE4 dysfunction is associated with diseases such as depression, Alzheimer's disease, and asthma, and its inhibitors have shown potential value in neuroprotection and antidepressant effects.

Picture

Western Blot

WB result of pan PDE4 Recombinant Rabbit mAb
Primary antibody: pan PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Low expression control: K562 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 60-100 kDa

WB result of pan PDE4 Recombinant Rabbit mAb
Primary antibody: pan PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 60-100 kDa

WB result of pan PDE4 Recombinant Rabbit mAb
Primary antibody: pan PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 60-100 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human brain. Anti-pan PDE4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brain. Anti-pan PDE4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-pan PDE4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.