WB result of pan Brd4 Recombinant Rabbit mAb
Primary antibody: pan Brd4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 Āµg
Lane 2: HeLa whole cell lysate 20 Āµg
Lane 3: K-562 whole cell lysate 20 Āµg
Lane 4: MCF7 whole cell lysate 20 Āµg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 90-140 kDa
pan Brd4 Recombinant Rabbit mAb (S-6208)
pan Brd4 Recombinant Rabbit mAb (S-6208)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Brd4 |
| Synonyms | Bromodomain-containing protein 4; HUNK1; BRD4 |
| Location | Nucleus |
| Accession | O60885 |
| Clone Number | S-6208 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | 293T, HeLa, K-562, MCF7, NIH/3T3, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 Ā°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
BRD4 (bromodomain-containing protein 4) is a core member of the BET protein family and functions as a key "epigenetic reader," specifically recognizing and binding acetylated lysine residues on histones and transcription factors through its tandem bromodomains (BD1 and BD2), thereby regulating gene transcription at the chromatin level. The protein consists of approximately 1,400 amino acids and contains two N-terminal bromodomains, an extraterminal (ET) domain, a unique C-terminal motif (CTM), and multiple intrinsically disordered regions (IDRs). The functions of BRD4 extend far beyond acetyl-lysine recognition: its C-terminal CTM region recruits the positive transcription elongation factor b (P-TEFb) complex (CDK9/CCNT1), phosphorylates the C-terminal domain of RNA polymerase II to drive transcriptional elongation, and BRD4 itself also possesses atypical kinase activity and histone acetyltransferase (HAT) activity. During the cell cycle, BRD4 remains bound to chromatin as a "mitotic bookmark," maintaining epigenetic memory and promoting transcriptional restart after mitosis. Recent studies have revealed that the intrinsically disordered regions of BRD4 confer upon it the capacity for liquid-liquid phase separation (LLPS), enabling it to form biomolecular condensates at super-enhancer regions, concentrate the transcriptional machinery (such as MED1 and Pol II), and drive high expression of oncogenes (such as c-MYC)āa property that also makes BRD4 a therapeutic target in various hematological malignancies and solid tumors. In addition, BRD4 participates in diverse physiological and pathological processes, including the DNA damage response, viral latency reactivation, neuroinflammatory regulation, and osteoarthritic cartilage degeneration. Targeting strategies directed at its bromodomains, ET domain, and condensate propertiesāincluding small-molecule inhibitors, PROTAC degraders, and peptide-based disruptorsāare emerging as frontier directions in drug development.
Picture
Picture
Western Blot
WB result of pan Brd4 Recombinant Rabbit mAb
Primary antibody: pan Brd4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 Āµg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 90-140 kDa
WB result of pan Brd4 Recombinant Rabbit mAb
Primary antibody: pan Brd4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 Āµg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 90-140 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-pan Brd4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
