WB result of PAM Recombinant Rabbit mAb
Primary antibody: PAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NCI-H69 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 110 kDa
PAM Recombinant Rabbit mAb (SDT-2995-30)
PAM Recombinant Rabbit mAb (SDT-2995-30)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PAM |
| Synonyms | Peptidyl-glycine alpha-amidating monooxygenase |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P19021 |
| Clone Number | SDT-2995-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | NCI-H69, U-2 OS |
| Predicted Reactivity | Covering most species |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
Background
Peptidylglycine α-amidating monooxygenase (PAM) is a copper-dependent, bifunctional enzyme that catalyzes the C-terminal amidation of peptide hormones and neuropeptides—an essential post-translational modification for their full biological activity—through two sequential catalytic domains, with its expression, localization, and trafficking tightly regulated by alternative splicing, mRNA-binding proteins, and dynamic endocytic recycling, and genetic variants in PAM have been linked to metabolic disorders such as type 2 diabetes and pituitary hormone dysfunction.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human medullary thyroid carcinoma. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human papillary thyroid carcinoma. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
