Flow cytometric analysis of mouse splenocytes labeling Pacific Blue Syrian Hamster IgG Isotype control at 1/20 dilution (1 μg) / (Right panel) compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Syrian Hamster IgG Isotype control
Pacific Blue Syrian Hamster IgG Isotype control
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Product Details
Product Details
Product Specification
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | FCM, ICFCM |
| Purification | Immunogen Affinity |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeling Pacific Blue Syrian Hamster IgG Isotype control at 1/20 dilution (1 μg) / (Right panel) compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
