Flow cytometric analysis of mouse CD8α expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD3 Antibody and either Pacific Blue Rat IgG1, λ Isotype Control (left panel) or SDT Pacific Blue Rat Anti- Mouse CD8α Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 583 (S0D0015), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Pacific Blue Rat Anti-Mouse CD8α Antibody (S-353-45)
Pacific Blue Rat Anti-Mouse CD8α Antibody (S-353-45)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD8α |
| Synonyms | T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; Lyt-2; Lyt2; Cd8a |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01731 |
| Clone Number | S-353-45 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,λ |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD8α is a crucial immune glycoprotein that serves as a co-receptor for the MHC I-peptide complex, facilitating the recognition of antigens presented by APCs (antigen-presenting cells) to T cells. It is a key component of the CD8 receptor complex, which is predominantly found on cytotoxic T lymphocytes (CTLs). CD8α can form either an α/β heterodimer with CD8β or an α/α homodimer, both of which play essential roles in CTL activation and function. By interacting with the TCR (T cell receptor) and MHC I molecules, CD8α helps recruit the LCK kinase, initiating multiple signaling pathways that drive the CTL-mediated immune response.
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