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Pacific Blue Rat Anti-Mouse CD2 Antibody (RM2-5)

Pacific Blue Rat Anti-Mouse CD2 Antibody (RM2-5)

Catalog Number: S0B8716 Application: FCM Reactivity: Ms Conjugation: Pacific Blue Brand: Starter
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Regular price $100 USD
Regular price Sale price $100 USD
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Product Details

Product Specification


Host Rat
Antigen CD2
Synonyms T-cell surface antigen CD2; LFA-2; LFA-3 receptor; Lymphocyte antigen 37 (Ly-37); T-cell surface antigen T11/Leu-5; Ly-37; Cd2
Location Cell membrane
Accession P08920
Clone Number RM2-5
Antibody Type Rat mAb
Isotype IgG2b,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein G
Concentration 0.5 mg/ml
Conjugation Pacific Blue
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1μg per million cells in 100μl volume Ms

Background

CD2, also known as the T-cell rosette receptor or LFA-2, is a type I membrane protein predominantly expressed on thymocytes and mature T cells. It functions as an adhesion molecule that binds to CD58 (LFA-3) on antigen-presenting cells (APCs), facilitating T-cell receptor (TCR) binding and signal transduction, which is crucial for T-cell activation and immune responses.

Picture

FC

Flow cytometric analysis of Mouse CD2 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD19 and either Pacific Blue™ Mouse IgG2b,k Isotype Control (left panel) or SDT Pacific Blue™ Rat Anti-Mouse CD2 antibody (right panel) at 2μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.