Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling SDT Pacific Blue Rabbit IgG Isotype Control Antibody at 1 μg/test (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Rabbit IgG Isotype Control Antibody (S-R058)
Pacific Blue Rabbit IgG Isotype Control Antibody (S-R058)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-R058 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
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FC
Flow cytometric analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labeling SDT Pacific Blue Rabbit IgG Isotype Control Antibody at 1 μg/test (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SDT Pacific Blue Rabbit IgG Isotype Control Antibody at 1 μg/test (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
