Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling Mouse IgG2b, κ Isotype Control (Pacific Blue Conjugate) at 1/20 (1 μg) dilution (Red) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-22 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:20 | |
| FCM | 1:20 |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Mouse IgG2b, κ Isotype Control (Pacific Blue Conjugate) at 1/20 (1 μg) dilution (Red) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
