Flow cytometric analysis of Mouse H-2Kb expression on C57BL/6 mouse splenocytes. Cells from the C57BL/6 mouse splenocytes (right) or BALB/c mouse splenocytes (left) were stained with either Pacific Blue™ Mouse IgG2a, κ Isotype Control (black line histogram) or SDT Pacific Blue™ Mouse Anti-Mouse H-2Kb Antibody (red line histogram) at 2μl/test, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Pacific Blue Mouse Anti-Mouse H-2Kb Antibody (AF6-88.5)
Pacific Blue Mouse Anti-Mouse H-2Kb Antibody (AF6-88.5)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | H-2Kb |
| Synonyms | H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K; H2-K1 |
| Location | Membrane |
| Accession | P01901 |
| Clone Number | AF6-88.5 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
MHC Class I (H-2Kb) is a 45 kDa type I transmembrane glycoprotein encoded within the mouse MHC locus on chromosome 17, functioning as the murine ortholog of human HLA-A; it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini, and once loaded it is transported to the cell surface where it surveys CD8⁺ cytotoxic T lymphocytes for the presence of viral, tumor or self-peptides, thereby orchestrating rapid immune elimination of aberrant cells while maintaining tolerance through engagement of inhibitory receptors such as Ly49 and KIR.
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