Flow cytometric analysis of Human CD203c expression on Human PBMC. Human PBMC (peripheral blood mononuclear cells) were stained with PE-Cy7 Mouse Anti-Human CD123 Antibody and either Pacific Blue Mouse IgG1, κ Isotype Control (Left panel) or SDT Pacific Blue Mouse Anti-Human CD203c Antibody (Right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Mouse Anti-Human CD203c Antibody (S-2916)
Pacific Blue Mouse Anti-Human CD203c Antibody (S-2916)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD203c |
| Synonyms | Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 1 Publication; E-NPP 3; NPP3; Alkaline phosphodiesterase I; Dinucleoside polyphosphatase; Nucleotide diphosphatase; Nucleotide pyrophosphatase (NPPase); Phosphodiesterase I beta (PD-Ibeta); Phosphodiesterase I/nucleotide pyrophosphatase 3; PDNP3; ENPP3 |
| Location | Secreted, Cell membrane |
| Accession | O14638 |
| Clone Number | S-2916 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD203c, also known as ectonucleotide pyrophosphatase/phosphodiesterase-3 (E-NPP3), is a type II transmembrane protein belonging to the ectonucleotide pyrophosphatase/phosphodiesterase family. It is specifically expressed on basophil-committed progenitor cells and mature basophils, and to a lesser extent on tissue mast cells. CD203c is rapidly upregulated on the cell surface upon basophil activation, such as through cross-linking of the IgE receptor, making it a valuable activation marker for these cells. It plays a role in regulating the inflammatory response by hydrolyzing ATP, which helps suppress chronic allergic inflammation. Additionally, CD203c has been identified as a potential biomarker for basophilia in diseases like chronic myeloid leukemia (CML), where its expression levels correlate with disease severity and treatment response.
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