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Pacific Blue Mouse Anti-Human CD144 Antibody (S-3026)

Pacific Blue Mouse Anti-Human CD144 Antibody (S-3026)

Catalog Number: S0B80318 Application: FCM Reactivity: Hu Conjugation: Pacific Blue Brand: Starter
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$85 USD
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Product Details

Product Specification


Host Mouse
Antigen CD144
Synonyms Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5
Location Cell membrane, Cell junction
Accession P33151
Clone Number S-3026
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Reactivity Hu
Positive Sample HUVEC
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Pacific Blue
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation .

Picture

FC

Flow cytometric analysis of CD144 expression on HUVEC (Human umbilical vein endothelial cell). Cells from HUVEC were stained with either Pacific Blue Mouse IgG1, κ Isotype Control (black line histogram) or SDT Pacific Blue Mouse Anti-Human CD144 Antibody (red line histogram) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.