Flow cytometric analysis of the major hematopoietic cell lineages expression on Balb/c mouse bone marrow cells were stained with PE/Cy7 Rat IgG Isotype Control (Left panel) or SDT PE-Cy7 Rat Anti-Mouse CD117 Antibody (S-R681) (Right panel, S0B5669) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Anti-Mouse Lineage Cocktail
Pacific Blue Anti-Mouse Lineage Cocktail
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$670 USD
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$670 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse Lineage Cocktail |
| Synonyms | Lin-1 |
| Location | Membrane |
| Antibody Type | Rat mAb |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ anti-Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G, clone S-R481; anti mouse CD11b, clone S-R574; anti-mouse CD45R/B220, clone S-R519; anti-mouse TER 119/Erythroid cells, clone S-R517.
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