WB result of PABP Recombinant Rabbit mAb
Primary antibody: PABP Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
PABP Recombinant Rabbit mAb (S-1744-42)
PABP Recombinant Rabbit mAb (S-1744-42)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PABP |
| Synonyms | Polyadenylate-binding protein 1; PABP-1; Poly(A)-binding protein 1; PABPC1; PAB1; PABP1; PABPC2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P11940 |
| Clone Number | S-1744-42 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HT-29, LNCaP, MCF7, NIH/3T3, mouse brain, mouse testis, PC-12, rat brain, rat testis |
| Predicted Reactivity | Xe, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms |
Background
Poly(A)-binding protein (PABP) is a widely conserved RNA-binding protein in eukaryotic cells that binds to the poly(A) tail of mRNA through its RNA recognition motifs (RRMs). PABP plays a crucial role in translation regulation, mRNA stability, and degradation. By interacting with the translation initiation factor eIF4G, PABP enhances ribosomal recruitment and translation initiation efficiency while protecting the poly(A) tail from degradation. Additionally, PABP is involved in mRNA storage, nucleocytoplasmic transport, nonsense-mediated mRNA decay (NMD), and miRNA-mediated translational repression. During cellular stress and viral infections, PABP's function can be modulated; for example, the influenza virus NS1 protein can bind to PABP to inhibit its activity. Dysregulation of PABP expression and function has been implicated in various diseases, including neurodegenerative disorders, metabolic diseases, and cancer.
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Picture
Western Blot
WB result of PABP Recombinant Rabbit mAb
Primary antibody: PABP Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
WB result of PABP Recombinant Rabbit mAb
Primary antibody: PABP Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-PABP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PABP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PABP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-PABP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-PABP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
