p58IPK/DNAJC3 Recombinant Rabbit mAb (S-4816-205)

Catalog Number: S0B60538 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	p58IPK/DNAJC3
Immunogen	Synthetic Peptide
Location	Endoplasmic reticulum
Accession	Q13217
Clone Number	S-4816-205
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, BxPC-3, Hep G2, HT-29, PC-3, NIH/3T3, mouse testis, rat testis
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

P58IPK (also known as DNAJC3 or ERdj6) is an endoplasmic reticulum-resident DnaJ family co-chaperone that was initially discovered as a cytosolic inhibitor of the PKR kinase during influenza virus infection, and was later shown to be induced by endoplasmic reticulum stress and to participate in negative feedback regulation of the unfolded protein response (UPR) by inhibiting PERK activity. The protein consists of an N-terminal endoplasmic reticulum-targeting signal sequence, an N-terminal domain containing nine tetratricopeptide repeat (TPR) motifs, and a conserved C-terminal J domain. Its J domain is attached to the end of the elongated TPR domain via a flexible linker, with the HPD motif located at the protein's edge, approximately 100 Å away from the substrate-binding site. P58IPK possesses dual functions as both a molecular chaperone and a co-chaperone: the hydrophobic groove of its TPR domain can directly bind unfolded or misfolded protein substrates, and it then binds the endoplasmic reticulum Hsp70 homolog BiP through its J domain, stimulating BiP's ATPase activity and promoting substrate transfer to BiP.

Picture

Western Blot

WB result of p58IPK/DNAJC3 Recombinant Rabbit mAb
Primary antibody: p58IPK/DNAJC3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Hep G2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 54 kDa

WB result of p58IPK/DNAJC3 Recombinant Rabbit mAb
Primary antibody: p58IPK/DNAJC3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 54 kDa

WB result of p58IPK/DNAJC3 Recombinant Rabbit mAb
Primary antibody: p58IPK/DNAJC3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 54 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-p58IPK/DNAJC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.