The P53[Y220C] activity was detected using HTRF technology. The reaction was performed by incubating the P53[Y220C] protein, dsDNA and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
p53[Y220C] His Tag Protein
p53[Y220C] His Tag Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53 |
| Accession | P04637-1 |
| Amino Acid Sequence | S94-T312 |
| Expression System | E.coli |
| Molecular Weight | 26528.0 |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20mM Tris, pH7.4, 150mM NaCl, 5mM DTT, 10uM Zn2+, 10% glycerol |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg P53[Y220C]
2 3 μg P53[Y220C]
