p53 DINP1 Recombinant Rabbit mAb

Catalog Number: S0B60743 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	p53 DINP1
Synonyms	Tumor protein p53-inducible nuclear protein 1; Stress-induced protein; p53-dependent damage-inducible nuclear protein 1 (p53DINP1); P53DINP1; SIP; TP53INP1
Location	Cytoplasm, Nucleus
Accession	Q96A56
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Hep G2, RAW264.7, mouse lung, C6, rat lung
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:100	Hu, Ms, Rt

Background

p53DINP1, also known as TP53INP1, is a p53-inducible nuclear protein that functions as a stress-responsive tumor suppressor. It is upregulated by DNA damage and cellular stress, and promotes p53-dependent apoptosis by facilitating p53 phosphorylation at Ser46 and inducing p53AIP1. p53DINP1 also acts as a dual regulator of transcription and autophagy, shuttling between the nucleus and autophagosomes where it interacts with LC3/GABARAP family proteins to support autophagic cell death.

Picture

Western Blot

WB result of p53 DINP1 Recombinant Rabbit mAb
Primary antibody: p53 DINP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Hep G2 whole cell lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 31 kDa
This blot was developed with high sensitivity substrate

WB result of p53 DINP1 Recombinant Rabbit mAb
Primary antibody: p53 DINP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raw 264.7 whole cell lysate 25 µg
Lane 2: mouse lung lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 31 kDa
This blot was developed with high sensitivity substrate

WB result of p53 DINP1 Recombinant Rabbit mAb
Primary antibody: p53 DINP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 25 µg
Lane 2: rat lung lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 31 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-p53 DINP1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-p53 DINP1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-p53 DINP1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-p53 DINP1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.