WB result of p300 Recombinant Rabbit mAb
Primary antibody: p300 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 264 kDa
Observed MW: 280 kDa
The band below the target band is protein degradation
p300 Recombinant Rabbit mAb (S-2612-122)
p300 Recombinant Rabbit mAb (S-2612-122)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p300 |
| Synonyms | Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300; EP300 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q09472 |
| Clone Number | S-2612-122 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | 293T, Jurkat, C2C12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
Background
p300 is a 2,414-residue human transcriptional coactivator that contains multiple conserved domains—bromodomain, CH1–CH3, HAT, KIX, and ZZ-type zinc finger—that enable it to serve as a histone acetyltransferase and a scaffold bridging sequence-specific DNA-binding factors with chromatin-remodeling machinery; by acetylating histones H3 and H4 as well as non-histone substrates such as p53 and NF-κB, it opens local chromatin structure and potentiates transcription, while its own activity is tightly regulated through phosphorylation, auto-acetylation, and competitive binding with viral oncoproteins like E1A or cellular partners like CBP; because it integrates proliferative, differentiation, and DNA-damage signals, p300 is indispensable during embryogenesis and is frequently altered—mutated, deleted, or translocated—in cancers, making it both a tumor suppressor and, in certain contexts, an oncogenic driver, and its HAT activity is pharmacologically targeted by small-molecule inhibitors under investigation for cancer therapy.
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Picture
Western Blot
WB result of p300 Recombinant Rabbit mAb
Primary antibody: p300 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 264 kDa
Observed MW: 280 kDa
The band below the target band is protein degradation
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in 293T cells. Anti- p300 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
