IF shows positive staining in paraffin-embedded human tonsil. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
p27 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-R350)
p27 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-R350)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p27 |
| Synonyms | Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b, KIP-1 |
| Location | Cytoplasm, Nucleus |
| Accession | P46414 |
| Clone Number | SDT-R350 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:50 | Hu |
Background
p27 Kip1 is a member of Cip/Kip family of proteins, leading to cell cycle arrest in G1 phase. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung. In pathology, it can be used for the identification of thyroid adenoma and thyroid follicular carcinoma.
Picture
Picture
Immunofluorescence
IF shows positive staining in paraffin-embedded human testis. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
