WB result of OTX2 Recombinant Rabbit mAb
Primary antibody: OTX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: NTERA-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 35 kDa
OTX2 Recombinant Rabbit mAb (S-2095-8)
OTX2 Recombinant Rabbit mAb (S-2095-8)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | OTX2 |
| Synonyms | Homeobox protein OTX2; Orthodenticle homolog 2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P32243 |
| Clone Number | S-2095-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms |
| Positive Sample | NTERA-2, normal mouse E14 foetal brain |
| Predicted Reactivity | Ys, Zf |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000 | Hu, Ms |
| IHC-P | 1:5000 | Ms |
| IF | 1:500 | Ms |
Background
OTX2 protein, also known as orthodenticle homeobox 2, is a transcription factor encoded by the OTX2 gene and belongs to the homeobox-containing gene family. It plays crucial roles in various developmental processes, particularly in the formation and function of the central nervous system. OTX2 is highly expressed in the brain, including regions such as the cerebral cortex and cerebellum. This protein is also involved in the development of the retina, where it is expressed in photoreceptors and bipolar cells. Additionally, OTX2 has been implicated in non-cell-autonomous activities, meaning it can influence neighboring cells through diffusion and interaction. Abnormalities in the OTX2 gene have been associated with conditions like microphthalmia and pituitary hormone deficiency. Recent studies also suggest a potential link between OTX2 and major depressive disorders.
Picture
Picture
Western Blot
WB result of OTX2 Recombinant Rabbit mAb
Primary antibody: OTX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: normal mouse E14 foetal brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse E14.5 choroid plexus. Anti-OTX2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse E14.5 retina. Anti-OTX2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse E14.5 retina. Anti- OTX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse E14.5 retina. Anti- OTX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse E14.5 retina. Anti- OTX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
