Negative control: IHC shows negative staining in paraffin-embedded human small cell lung cancer. Anti-OTP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
OTP Recombinant Rabbit mAb (SDT-2002-175)
OTP Recombinant Rabbit mAb (SDT-2002-175)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | OTP |
| Synonyms | Homeobox protein orthopedia; OTP |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q5XKR4 |
| Clone Number | SDT-2002-175 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Predicted Reactivity | Ms, Zf |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
Orthopedia homeobox protein (OTP) is a nuclear transcription factor encoded on chromosome 5q14.1 that belongs to the homeobox family and is indispensable for embryonic neurodevelopment ; in clinical oncology it serves as a highly sensitive and specific immunohistochemical marker that strongly stains typical pulmonary carcinoids while remaining negative in most non-lung well-differentiated neuroendocrine tumors, with negative or weak nuclear OTP expression correlating with worse prognosis in carcinoid patients .
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human endometrial carcinoma. Anti-OTP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-OTP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-OTP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
