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    Organoid Retrieval Solution

    Organoid Retrieval Solution

    Catalog Number: abs9920 Brand: Absin
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    $238 USD
    $238 USD
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    Product Details

    Product Specification

    Usage 1. Organoid collection: Under the condition of retaining the original culture medium in the well plate (or removing the culture medium and adding an equal volume of basal culture medium), gently scrape off the matrix gel and organoid mixture with the tip of the pipette tip (do not blow vigorously, just collect), transfer to 1.5ml, and then let stand for 2-10min (or centrifuge 100g at low speed for 3min).
    2. Add recovery solution: Retain the matrix gel and organoid mixture. After discarding the supernatant, add 10 times the volume of organoid recovery solution according to the precipitation amount (recommended precipitation amount of 50 μL/tube). Shake gently to allow the precipitate to fully contact the recovery solution.
    3. Incubation: Incubate on ice for 30 minutes. During the incubation process, shake gently 3-4 times every 5 minutes.
    4. Centrifuge: Centrifuge 250g for 3 minutes, discard the supernatant.
    5. Washing: Add pre-cooled epithelial organoid-specific basic medium to wash the organoids once, and centrifuge to discard the supernatant to obtain organoid precipitate.  
    6. The recovered organoids can be used for downstream experiments and biochemical analysis.
    Description This product is a ready-to-use solution for recovering intact organoids from matrix glue. The organoid recovery solution can rapidly dissolve the matrix gum in an environment of 2-8°C without causing damage to the shape or structure of the organoid throughout the process. Organoids obtained from organoid recovery solution can be used for organoid passage, cryopreservation, and other related experimental studies, such as RNA and protein extraction, organoid section staining, etc.
    Storage Temp. Store at 2-8 °C, protected from light, shelf life 12 months.