Organoid Digestion Solution
Organoid Digestion Solution
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| Usage | 1. Pipette off the culture medium, add 1 ml of 4℃ organoid buffer B to each well and leave for 2 min. Pipette and gently blow the matrix gel, collect it in a 15 ml centrifuge tube, and let it stand at 4℃ for 10 min. (One set per 6-8 wells). 2. Centrifuge for 5 min to discard the supernatant, add an appropriate amount of organoid passage digestion solution to digest for 2-3 min, add 5 times the volume of organoid buffer to terminate the digestion, centrifuge for 5 min to discard the mixture, add organoid buffer B, resuspend and transfer into a 5 ml centrifuge tube, centrifuge 300 g for 5 min to discard the liquid for step 3.Note:① The digestion process must be carefully monitored during this operation to avoid excessive digestion. During digestion, a pipette was used to blow and mix to aid digestion. A small amount of digestion suspension can also be taken in real time to observe the digestion under a microscope. When a large number of single cells or cell clusters with a diameter of less than 50 um are observed, the digestion can be considered complete. ② Add at least 5 times the volume of organoid buffer to the suspension after confirming the completion of digestion for dilution to terminate the digestion. 3. The organoid precipitate obtained in the previous step can be used for subsequent experimental operations such as organoid culture and cryopreservation. Note: Organoid passage digestion solution can be used in conjunction with organoid culture medium. |
| Description | Organoid Digestion Solution can be applied to the routine passage of organoids of various mammalian (such as human, mouse) origin. It can separate the organoids from the matrix gel and gently digest them into small cell clusters or single cells, while maintaining their growth viability after passage. |
| Storage Temp. | -20°C, shelf life 12 months. |