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    Organoid Cryopreservation Solution

    Organoid Cryopreservation Solution

    Catalog Number: abs9519 Brand: Absin
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    $111 USD
    $111 USD
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    Product Details

    Product Specification

    Usage 1. Add pre-cooled (2-8℃) organoid cryopreservation solution to the recovered organoids (or cells), blow and mix well, and then quickly transfer to the cell cryopreservation tube (the volume of cell suspension in the cryopreservation tube should be greater than 0.5ml). Note: It is recommended to cryopreserve 1x10 per 1mL of cryopreservation solution.3-1x107An organoid with a number of cells or a corresponding number of cells.
    2. Place the cryopreservation tube into the cell cryopreservation cooling box (the cooling box must be equilibrated to room temperature or 4℃ in advance), and then immediately place the cooling box into a -80℃ ultra-low temperature refrigerator; The cryopreservation tubes can also be subjected to artificial gradient cooling treatment, such as standing at 4℃ for 10 minutes, storing at -20℃ for 1 hour, and storing at -80℃ overnight.
    3. The next day or 12 hours later, transfer the cryopreservation tubes to liquid nitrogen for long-term storage under low temperature conditions (not higher than -70℃, dry ice or the original cooling box is recommended).

    If the organoid (or cell) is resuscitated, the following steps can be performed:
    1. Transfer 10 mL of organoid culture buffer B to a 15 mL centrifuge tube.
    2. Remove the frozen organoid cells from the liquid nitrogen tank and quickly place them in a 37℃ water bath to thaw.
    3. During the water bath thawing process, the freezing tube should be gently shaken to ensure that the frozen solution is completely thawed within 1-2 minutes.
    4. Quickly transfer the lysed organoid cells to a 15 mL centrifuge tube, gently blow 6-8 times with a pipette, centrifuge 300 g for 5 minutes, then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid buffer B, resuspend, transfer to a 1.5 ml centrifuge tube, 300 g, and centrifuge for 5 min.
    5. Resuspend the matrix gel. Place 25 μL of matrix gel per well in a 24-well cell culture plate, place it in the incubator for 10-15 minutes to form gel, and add 500 μL of organoid medium A.

    Note: Organoid cryopreservation solution (abs9519) can be used with organoid culture medium.
    Description Organoid Cryopreservation mediums can be used for the long-term Cryopreservation of organoids and cell lines derived from various mammals (such as humans and mice).
    Storage Temp. -20°C, shelf life 12 months.