HC&GFR OrganoGel Phenol red free

The basement membrane is a layer of stromal membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from a basement membrane component extracted from mouse tumor tissue. The main component of that matrix glue are laminin, collagen IV, heparan sulfateproteoglycans (Kleinman et al. 1986). At the same time, the matrix gel also contains various growth factors, such as epidermal growth factor, nerve growth factor (Vukicevic et al 1992)

PRODUCT CHARACTERISTICS: This product is liquid at 4°C, but is gel when heated to 37°C. After the matrix glue has solidified, it is re-placed at 4°C overnight and the matrix glue can be liquefied again.

The matrix glue begins to solidify at temperatures above 10°C., so try to handle the matrix glue on ice. In order to avoid the effect of enzymes on organoids during organoid passage, organoids can be released from the matrix gel by slowly blowing the matrix gel directly with 4°C pre-cooled basal medium.