HC&GFR OrganoGel Phenol red free

The basement membrane is a layer of matrix membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from basement membrane components extracted from mouse tumor tissue. The main components of the matrix glue are: laminin, collagen IV, heparan sulfateproteoglycans (Kleinman et al. 1986). At the same time, the matrix glue also contains a variety of growth factors, such as epidermal growth factor, nerve growth factor (Vukicevic et al 1992)

Product Characteristics: This product is liquid at 4°C, but gels when heated to 37°C. After the matrix gum has set, it is placed back at 4° C. overnight and the matrix gum can be liquefied again.

This matrix begins to solidify at temperatures above 10°C, so it is best to handle the matrix on ice. During organoid passage, in order to avoid enzymes affecting the organoids, the matrix gel can be slowly blown with 4°C pre-cooled basal medium to release the organoids from the matrix gel.