Product Details
Product Details
Product Specification
| Usage | See Operation Manual for details |
| Description | The basement membrane is a layer of stromal membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from a basement membrane component extracted from mouse tumor tissue. The main component of that matrix glue are laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, the matrix gel also contains a variety of growth factors, such as epidermal growth factor EFG, platelet-derived growth factor PDGF, nerve growth factor NGF, basic fibroblast growth factor FGF-2, beta-transforming growth factor TGF-beta, and insulin-like growth factor ILGF (Vukicevic et al. 1992). PRODUCT CHARACTERISTICS: This product is liquid at 4°C, but is gel when heated to 37°C. After the matrix glue has solidified, it is re-placed at 4°C overnight and the matrix glue can be liquefied again. |
| Source | Components of mouse tumor basement membrane |
| General Notes | The matrix glue begins to solidify at temperatures above 10°C., so try to handle the matrix glue on ice. In order to avoid the effect of enzymes on organoids during organoid passage, organoids can be released from the matrix gel by slowly blowing the matrix gel directly with 4°C pre-cooled basal medium. |
| Storage Temp. | It is recommended that the user divide the packaging according to the single dosage after the first melt and store it in the refrigerator at-80°C for 2 years. |
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Immunohistochemistry

