Comparison of organoids of colorectal cancer cultured with different brands of matrix gel (Figure 1)
Product Details
Product Details
Product Specification
| antigen | SFRS9 |
| Clonality | Polyclonal Antibody |
| Usage | See the operating manual for details. |
| Immunogen | A synthesized peptide derived from Human SRSF9(Accession Q13242), corresponding to amino acid residues Y77-P105. |
| Species Reactivity | Human;Mouse;Rat |
| UniProt | Q13242 |
| Purification | The antiserum was purified by peptide affinity chromatography using SulfoLinkâ„¢ Coupling Resin . |
| Specificity | SFRS9 Antibody detects endogenous levels of total SFRS9. |
| Post-translational modifications | Extensively phosphorylated on serine residues in the RS domain. |
| Host | Rabbit |
| Description | The basement membrane is a layer of matrix membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from basement membrane components extracted from mouse tumor tissue. The main components of the matrix glue are: laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, the matrix glue also contains a variety of growth factors, such as epidermal growth factor EFG, platelet-derived growth factor PDGF, nerve growth factor NGF, basic fibroblast growth factor FGF-2, B-type transforming growth factor TGF-beta and insulin-like growth factor ILGF (Vukicevic et al. 1992). Product characteristics: This product is liquid at 4°C, but gels when heated to 37°C. After the matrix gum has set, it is placed back at 4° C. overnight and the matrix gum can be liquefied again. |
| Source | mouse tumor basement membrane components |
| page_application | WB;IHC;ELISA |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
| tissuespecificity | Expressed at high levels in the heart, kidney, pancreas and placenta, and at lower levels in the brain, liver, lung and skeletal muscle. |
| Background | Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10. |
| General Notes | This matrix begins to solidify at temperatures above 10°C, so it is best to handle the matrix on ice. During organoid passage, in order to avoid enzymes affecting the organoids, the matrix gel can be slowly blown with 4°C pre-cooled basal medium to release the organoids from the matrix gel. |
| Storage Temp. | It is recommended that users repackage the product according to a single dose after the first thaw and store it in a refrigerator at -80°C. It has a shelf life of 2 years. |
| Applications | It is suitable for studies related to growth factors, signaling pathways, etc., or studies that require highly defined basement membranes. |
| Cellular localization | Nucleus; |
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Immunohistochemistry


Comparison of organoids of colorectal cancer cultured with different brands of matrix gel (Figure 2)

