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GFR OrganoGel with Phenol red

GFR OrganoGel with Phenol red

Catalog Number: abs9494 Reactivity: Human;Mouse;Rat Conjugation: Brand: Absin
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Regular price $310 USD
Regular price Sale price $310 USD
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Product Details

Product Specification

antigen SFRS9
Clonality Polyclonal Antibody
Usage See Operation Manual for details
Immunogen A synthesized peptide derived from Human SRSF9(Accession Q13242), corresponding to amino acid residues Y77-P105.
Species Reactivity Human;Mouse;Rat
UniProt Q13242
Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Specificity SFRS9 Antibody detects endogenous levels of total SFRS9.
Post-translational modifications Extensively phosphorylated on serine residues in the RS domain.
Host Rabbit
Description The basement membrane is a layer of stromal membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from a basement membrane component extracted from mouse tumor tissue. The main component of that matrix glue are laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, the matrix gel also contains a variety of growth factors, such as epidermal growth factor EFG, platelet-derived growth factor PDGF, nerve growth factor NGF, basic fibroblast growth factor FGF-2, beta-transforming growth factor TGF-beta, and insulin-like growth factor ILGF (Vukicevic et al. 1992).
Product Characteristics:
This product is liquid at 4°C but gel when heated to 37°C. After the matrix glue has solidified, it is re-placed at 4°C overnight and the matrix glue can be liquefied again.
Source Components of mouse tumor basement membrane
page_application WB;IHC;ELISA
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
tissuespecificity Expressed at high levels in the heart, kidney, pancreas and placenta, and at lower levels in the brain, liver, lung and skeletal muscle.
Background Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.
General Notes The matrix glue begins to solidify at temperatures above 10°C., so try to handle the matrix glue on ice. In order to avoid the effect of enzymes on organoids during organoid passage, organoids can be released from the matrix gel by slowly blowing the matrix gel directly with 4°C pre-cooled basal medium.
Storage Temp. It is recommended that the user divide the packaging according to the single dosage after the first melt and store it in the refrigerator at-80°C for 2 years.
Applications It is suitable for studies related to growth factors, signaling pathways, etc., or studies requiring basement membranes with highly defined components.
Cellular localization Nucleus;

Picture

Immunohistochemistry

Comparison of organoids of intestinal cancer cultured by different brands of matrix gel

Comparison of organoids of intestinal cancer cultured by different brands of matrix gel Figure 2